mouse melanoma cell line b16 f10  (ATCC)

Journal: bioRxiv

Article Title: ME1 Programs Latent Effector Capacity and Grounds a Mathematical Model of Reversible T Cell Exhaustion

doi: 10.64898/2026.05.05.722814

Figure Lengend Snippet: (A) Schematic illustrating the generation of CD8-specific ME1 transgenic (ME1 Tg) mice. (B) Representative flow cytometry showing tdTomato expression as a surrogate for ME1 overexpression in CD8 + tumor-infiltrating lymphocytes (TILs). (C–D) Average growth curves of B16F10 tumors in control and CD8-ME1 Tg mice treated with PBS (C) or combined anti-PD-1/anti-PD-L1 antibodies (100 μg each per dose) administered every other day starting on day 6 (arrow) after tumor implantation. Tumor growth was analyzed by two-way ANOVA (D, n = 6, **P < 0.01). (E) Tumor sizes were measured at endpoint. Data were analyzed using an unpaired two-tailed t test (n = 4–8, ***P < 0.001). One of two independent experiments is shown. (F) Flow cytometric analysis of granzyme B (GZMB) protein expression in CD8 + TILs cells from B16F10 tumors in ME1 Tg and control mice on day 12. Data were analyzed using an unpaired two-tailed Student’s t test ( * P< 0.05; n = 5 mice per group).

Article Snippet: B16F10 murine melanoma cell line was purchased from ATCC (CRL-6475) and cultured using DMEM complete medium.

Techniques: Transgenic Assay, Flow Cytometry, Expressing, Over Expression, Control, Tumor Implantation, Two Tailed Test

Journal: Molecules

Article Title: Synthesis, Spectroscopy Characterization and Biological Evaluation of La(III), Eu(III) and Gd(III) Complexes with Ampicillin: In Vitro Antimicrobial, Cytotoxic and Antiproliferative Activities and Theoretical Frameworks

doi: 10.3390/molecules31091465

Figure Lengend Snippet: Antiproliferative activity in vitro of the free ligand (NaL), the lanthanide chlorides (LnCl 3 , Ln = La(III), Eu(III) and Gd(III)), and the synthesized complexes, LaL = [La 2 (L)(Cl) 5 (H 2 O) 2 ] , EuL = [Eu 2 (L)(Cl) 5 (H 2 O) 5 ] and GdL = [Gd 2 (L)(Cl) 5 (H 2 O) 5 ] against the following cell lines: ( A ) B16-F10, ( B ) U251, ( C ) MCF7 and ( D ) NIH-3T3 exposed to different concentrations (0.25, 2.5, 25 and 250 μg·mL −1 ) for 48 h.

Article Snippet: Cell lines MCF7 (ATCC ® –HTB-22TM, breast carcinoma) and U251 (glioma) were acquired by Roswell Park Memorial Institute and cell lines B16-F10 (ATCC ® -CRL-6475TM, murine melanoma) and NIH-3T3 (ATCC ® -CRL-1658TM, murine fibroblast) (UNICAMP, BR).

Techniques: Activity Assay, In Vitro, Synthesized